Library creation device for single cell expression analysisChromium iX
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Overview
This device uses the droplet method to prepare libraries for high-throughput (~20,000 cells) single-cell gene expression analysis at medium resolution (2,000 genes/cell). A dedicated reagent kit from 10X Genomics is used depending on the application. After introducing a sample dispersed in single cells into this device to create a gel bead-in-emulsion, we prepare a library for next-generation sequencer analysis, perform gene sequencing with approximately 20,000 reads/cell, and generate analysis data. get.
| Name (abbreviation) | Library creation device for single cell expression analysis（Chromium iX） |
|---|---|
| Model number (manufacturer) | 10X Genomics |
| Application | Single cell gene expression analysis, single cell gene expression analysis, Flex (fixed sample, FFPE), VDJ repertoire analysis (immune cell profiling), ATACseq |
| Installation location | Imabari Campus Teaching Hospital Building 304 (Clinical Laboratory) |
| Equipment manager | Department of Veterinary Medicine Kohei Saeki |
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